This past Tuesday I dissected a chicken embryo... I extracted the heart and cut it into small pieces. Then I placed a single piece into an individual well of a 6 well cell culture plate, and added some DMEM + antibiotic media. A few of the chunks I added to an enzyme solution called trypsin which digests the extracellular protein scaffold that holds cells together, then I added some of this cell solution to a well in the plate and added the same growth media.
The next day the heart explants (chunks) were still beating:
Beating chick heart explant
Beating chick heart trypsinized cells
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